mimic negative control Search Results


mimics, inhibitor negative controls hsa-mir-1306-3p  (Ribobio co)
90
Ribobio co mimics, inhibitor negative controls hsa-mir-1306-3p
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Mimics, Inhibitor Negative Controls Hsa Mir 1306 3p, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/pmc06704702-112-7-11?v=Ribobio+co
Average 90 stars, based on 1 article reviews
mimics, inhibitor negative controls hsa-mir-1306-3p - by Bioz Stars, 2026-08
90/100 stars
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negative control mimic  (Ribobio co)
90
Ribobio co negative control mimic
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Negative Control Mimic, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/pm30365049-48-15-31?v=Ribobio+co
Average 90 stars, based on 1 article reviews
negative control mimic - by Bioz Stars, 2026-08
90/100 stars
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negative control (nc) mimics  (Shanghai GenePharma)
90
Shanghai GenePharma negative control (nc) mimics
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Negative Control (Nc) Mimics, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/pmc08661150-87-7-17?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
negative control (nc) mimics - by Bioz Stars, 2026-08
90/100 stars
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mimics negative control precursor (nc)  (Shanghai GenePharma)
90
Shanghai GenePharma mimics negative control precursor (nc)
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Mimics Negative Control Precursor (Nc), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/pm27878247-49-4-20?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
mimics negative control precursor (nc) - by Bioz Stars, 2026-08
90/100 stars
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50 nmol/l mimic-negative control (mimic-nc)  (Ribobio co)
90
Ribobio co 50 nmol/l mimic-negative control (mimic-nc)
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
50 Nmol/L Mimic Negative Control (Mimic Nc), supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/pmc06961604-83-19-5?v=Ribobio+co
Average 90 stars, based on 1 article reviews
50 nmol/l mimic-negative control (mimic-nc) - by Bioz Stars, 2026-08
90/100 stars
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mirna negative control mir01102-1-1  (Ribobio co)
90
Ribobio co mirna negative control mir01102-1-1
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Mirna Negative Control Mir01102 1 1, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/pmc10767360-112-17-21?v=Ribobio+co
Average 90 stars, based on 1 article reviews
mirna negative control mir01102-1-1 - by Bioz Stars, 2026-08
90/100 stars
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negative control mimics or inhibitor  (Ribobio co)
90
Ribobio co negative control mimics or inhibitor
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Negative Control Mimics Or Inhibitor, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/pm24446471-89-4-5?v=Ribobio+co
Average 90 stars, based on 1 article reviews
negative control mimics or inhibitor - by Bioz Stars, 2026-08
90/100 stars
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single-strand mimics or negative control of mimics (mimics-nc)  (Ribobio co)
90
Ribobio co single-strand mimics or negative control of mimics (mimics-nc)
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Single Strand Mimics Or Negative Control Of Mimics (Mimics Nc), supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/10__2147_slash_cmar__s363629-60-12-14?v=Ribobio+co
Average 90 stars, based on 1 article reviews
single-strand mimics or negative control of mimics (mimics-nc) - by Bioz Stars, 2026-08
90/100 stars
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mimic negative control (nc)  (Promega)
90
Promega mimic negative control (nc)
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Mimic Negative Control (Nc), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/10__2147_slash_ijgm__s272996-98-17-6?v=Promega
Average 90 stars, based on 1 article reviews
mimic negative control (nc) - by Bioz Stars, 2026-08
90/100 stars
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negative control mirna mimics pmir-control  (GenScript corporation)
90
GenScript corporation negative control mirna mimics pmir-control
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Negative Control Mirna Mimics Pmir Control, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/pm31059098-29-37-42?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
negative control mirna mimics pmir-control - by Bioz Stars, 2026-08
90/100 stars
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mimics negative control microntm mimics negative control#22  (Phalanx Biotech)
90
Phalanx Biotech mimics negative control microntm mimics negative control#22
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Mimics Negative Control Microntm Mimics Negative Control#22, supplied by Phalanx Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/pmc03420912-160-46-52?v=Phalanx+Biotech
Average 90 stars, based on 1 article reviews
mimics negative control microntm mimics negative control#22 - by Bioz Stars, 2026-08
90/100 stars
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sirnas, mir-1253 mimics, mir-1253 inhibitors and corresponding negative controls  (Ribobio co)
90
Ribobio co sirnas, mir-1253 mimics, mir-1253 inhibitors and corresponding negative controls
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Sirnas, Mir 1253 Mimics, Mir 1253 Inhibitors And Corresponding Negative Controls, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/pmc08349228-59-23-36?v=Ribobio+co
Average 90 stars, based on 1 article reviews
sirnas, mir-1253 mimics, mir-1253 inhibitors and corresponding negative controls - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of miR-1306-3p. a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01

Journal: Molecular Cancer

Article Title: Circular RNA circNHSL1 promotes gastric cancer progression through the miR-1306-3p/SIX1/vimentin axis

doi: 10.1186/s12943-019-1054-7

Figure Lengend Snippet: CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of miR-1306-3p. a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01

Article Snippet: The mimics, inhibitor and negative controls for hsa-miR-1306-3p were purchased from RiBoBio (Guangzhou, China).

Techniques: Expressing, Quantitative RT-PCR, Negative Control, Luciferase, Mutagenesis, Sequencing, Transfection, Agarose Gel Electrophoresis, Western Blot, Migration

MiR-1306-3p is down-regulated in gastric cancer tissues and correlated with the progression and poor prognosis. The expression of miR-1306-3p was detected by ISH with TMA including 54 paired gastric cancer tissues and paired normal tissues. a Represent images of miR-1306-3p expression in normal gastric mucosa and gastric cancer tissues with Stage II, Stage III, M0, M1, G1, G2, G3, pT2, pT3 and pT4. b The level of miR-1306-3p in gastric cancer tissues was significantly lower than in normal gastric tissues. c-f The level of miR-1306-3p in gastric cancer tissues with Stage II ( c ), M0 ( d ), well differentiation ( e ) and pT2 and pT3 ( f ) was significantly higher than these with Stage III, M1, moderate and poor differentiation and pT4. g - h Kaplan-Meier survival analysis (log-rank test) showed that gastric cancer patients with low miR-1306-3p expression have a lower OS and DFS than these with high miR-1306-3p expression ( p < 0.01). All data are presented as the mean ± SEM. ** p < 0.01

Journal: Molecular Cancer

Article Title: Circular RNA circNHSL1 promotes gastric cancer progression through the miR-1306-3p/SIX1/vimentin axis

doi: 10.1186/s12943-019-1054-7

Figure Lengend Snippet: MiR-1306-3p is down-regulated in gastric cancer tissues and correlated with the progression and poor prognosis. The expression of miR-1306-3p was detected by ISH with TMA including 54 paired gastric cancer tissues and paired normal tissues. a Represent images of miR-1306-3p expression in normal gastric mucosa and gastric cancer tissues with Stage II, Stage III, M0, M1, G1, G2, G3, pT2, pT3 and pT4. b The level of miR-1306-3p in gastric cancer tissues was significantly lower than in normal gastric tissues. c-f The level of miR-1306-3p in gastric cancer tissues with Stage II ( c ), M0 ( d ), well differentiation ( e ) and pT2 and pT3 ( f ) was significantly higher than these with Stage III, M1, moderate and poor differentiation and pT4. g - h Kaplan-Meier survival analysis (log-rank test) showed that gastric cancer patients with low miR-1306-3p expression have a lower OS and DFS than these with high miR-1306-3p expression ( p < 0.01). All data are presented as the mean ± SEM. ** p < 0.01

Article Snippet: The mimics, inhibitor and negative controls for hsa-miR-1306-3p were purchased from RiBoBio (Guangzhou, China).

Techniques: Expressing